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Creatinine Single Analyte Assay

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Creatinine Single Analyte Assay

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About Creatinine

Creatinine is a breakdown product of creatine produced by muscles. Creatinine is removed from the body by the kidneys, which filter it from the blood and release it into the urine. Serum creatinine has been used as a biomarker for acute kidney injury and chronic kidney disease. Urinary creatinine is used to test sample dilution, and the biomarker-to-creatinine ratio can be used to correct the fluctuation of urine volume.

Metabolomics reveals biological insights otherwise unseen. For a successful metabolomics study, both small molecule discovery and the ability to dig deeper into specific biomarkers of interest are needed to uncover actionable insights that propel new therapeutic developments. A specific combination of liquid chromatography-mass spectrometry (LC-MS) technology and biochemical expertise is required to identify this biomarker of interest and develop an assay that is sensitive enough to explore it fully.

At Metabolon, we understand the crucial role creatinine plays in kidney disease, and we’ve established best-in-class expertise for its detection. This creatinine assay can be used to track creatinine and enhance biological understanding across preclinical and clinical research.

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Creatinine Single Analyte Assay Details

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LLOQ*
Urine Plasma/Serum
50 µg/mL 2 µg/mL
*Lower Limit of Quantitation (LLOQ) varies for each sample type.

Analysis Method and Instrumentation:
LC-MS/MS (Agilent 1290 UHPLC/Sciex QTrap 5500)

Sample Type and Required Amounts
Sample Type Sample Requirements
Plasma/Serum ≥ 150 µL
Urine ≥ 150 µL

Others on request

Disclaimer: This panel is for Research Use Only and is not to be used for diagnostic purposes.

Delivering Absolute Quantification for Research and Biomarker Analysis

Our readily available or custom developed quantitative assays help you achieve your research and biomarker validation objectives with precise and fully validated methods. Our targeted assays and panels cover >1,000 metabolites and lipids across a wide range of biochemical classes, metabolic pathways, and physiological processes, and they can be customized to best fit any application.

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Creatinine Single Analyte Assay Applications

Kidney

More understanding is needed surrounding the normal and abnormal function of renal and urological tissue at a molecular and cellular level. Untargeted metabolomics can be applied to cultured cells, tissues from model organisms, and patient biopsies to discover metabolic drivers of processes such as cell death and malignant transformation. These insights can be translated into actionable biomarkers through follow-on targeted panels.
Learn more about metabolomics applications for renal and urological disorder research
kidney
Diabetes

Diabetes

Diabetes, a serious metabolic condition that results from the inability to regulate blood sugar levels, is a worldwide epidemic. According to the Diabetes Research Institute Foundation, more than 30 million Americans and 422 million people worldwide have been diagnosed with diabetes. Yet much remains unknown about how diabetes develops, why certain individuals are at increased risk, and whether the metabolic dysregulation can be corrected rather than merely managed.

Metabolomics enables simultaneous assessment of hundreds of compounds present in living systems, making it a critical tool that advances our understanding of disease mechanisms and opens doors to identify novel targets and treatments for conditions like diabetes.

Learn more about metabolomics applications for diabetes research

Big Insights with Metabolon

Cited in over 3,000 publications, we help scientists and manufacturers gain greater insight into their studies through metabolomics. See how our approach can become a successful part of your workflow.

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References

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