Indoles/Uremic Toxicity Targeted Panel

Metabolon Target

Indoles/Uremic Toxicity Targeted Panel

R 6 Metabolites

R Absolute Quantitation

R Rigorous Quality Control

R End-to-end Service

About Indoles and Uremic Toxicity

Uremic toxicity is the buildup of toxic analytes that are not able to be adequately filtered by the kidneys due to kidney injury or disease. Indole metabolites of tryptophan and the tyrosine metabolites p-cresol sulfate and 4-ethylphenyl sulfate are a key group of gut microbiota-derived uremic compounds. Their formation is the result of a complex interaction between diet, gut microbiota, and host. Increased production of these compounds by the gut microbiota and reduced clearance in kidney disease can lead to toxicity affecting nearly all systems in the body.

Metabolomics reveals biological insights otherwise unseen. For a successful metabolomics study, both small molecule discovery and the ability to dig deeper into specific biomarkers of interest are needed to uncover actionable insights that propel new therapeutic developments. A specific combination of liquid chromatography-mass spectrometry (LC-MS) technology and biochemical expertise is required to identify these biomarkers of interest and develop assays that are sensitive enough to explore them fully.

At Metabolon, we understand the crucial role indoles play in kidney disease, and we’ve established best-in-class expertise for their detection. This panel focuses on specific indoles and their metabolic pathways and can be used to track biomarkers and enhance biological understanding across preclinical and clinical research.

Indoles/Uremic Toxicity Targeted Panel Details

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LLOQ*
Metabolite Plasma/Serum
4-Ethylphenyl sulfate 1.00 ng/mL
p-Cresol sulfate 5.00 ng/mL
3-Indoxyl sulfate 10.0 ng/mL
Tryptophan 500 ng/mL
3-Indolelactic acid 10.0 ng/mL
3-Indolepropionic acid 7.50 ng/mL
*Lower Limit of Quantitation (LLOQ) varies for each sample type.

Analysis Method and Instrumentation:
LC-MS/MS (Agilent 1290 UHPLC/Sciex QTrap 5500)

Sample Type and Required Amounts
Sample Type Sample Requirements
Plasma/Serum 100 to 150 µL

Others on request

Disclaimer: This method is for Research Use Only and is not to be used for diagnostic purposes.

Delivering Absolute Quantification for Research and Biomarker Analysis

Our readily available or custom developed quantitative assays help you achieve your research and biomarker validation objectives with precise and fully validated methods. Our targeted assays and panels cover >1,000 metabolites and lipids across a wide range of biochemical classes, metabolic pathways, and physiological processes, and they can be customized to best fit any application.

Indoles/Uremic Toxicity Targeted Panel Applications

Kidney

The kidneys are essential for the elimination of waste products, the regulation of osmolality and blood pressure, pH homeostasis, and the secretion of certain hormones. Chronic kidney disease (CKD) often occurs as a result of other diseases, such as diabetes or high blood pressure. The Targeted Panel team at Metabolon has developed multiple assays that can provide insights into the function of renal and urological tissue, such as the Creatinine Assay, Indoles/Uremic Toxicity Panel, and the Metal Cofactors and Toxic Metals Assay.

kidney
gut health

Gut Health

Gut health is important for overall health. Alterations of the gut microflora can cause several types of gastrointestinal disorders (including irritable bowel syndrome and viral hepatitis. In addition, gut microflora has also been implicated to play a role in autoimmune disease, skin disorders, as well as in diabetes, and obesity. The unique ability for metabolomics to identify microbial metabolites and how they regulate pathways in the host makes the technology a valuable tool for understanding gastrointestinal and other metabolic disorders.

Neuroscience

Metabolomics provides valuable insight into the realm of brain science. Through dynamic bidirectional communication along the “gut-brain axis,” gut microbes collaborate with their hosts to regulate the development and function of the nervous system. For instance, in epilepsy, it is well-established that a low-carbohydrate, high-fat ketogenic diet (KD) can help treat refractory epilepsy, which affects more than a third of epileptic patients who don’t respond to existing anticonvulsive drugs. What scientists haven’t understood until recently is how this kind of diet translates to brain activity. The answer for this aspect of epilepsy lies in the gut microbiome. There are many other neurological disorders like Alzheimer’s disease, ALS, Parkinson’s disease and more. While so much remains to be understood about brain science, we do know that metabolomics is uniquely poised to understand the brain because of the ability of metabolites, small molecules, to cross the blood-brain barrier providing unique insights.
Neuroscience
covid

COVID

Recognized for our cutting-edge approach to metabolomics, Metabolon has been a valued resource for COVID-19 researchers worldwide. Our actionable metabolomic insights have fueled pivotal and high-profile studies like the National Institute of Allergy and Infectious Diseases (NIAID) IMPACC study research to improve understanding of high-risk patients and the Institute for Systems Biology research to improve understanding of high-risk patients. These and other metabolomics projects with actionable insights at Metabolon are helping get closer to the phenotype and pressing forward on COVID-19 answers.

Big Insights with Metabolon

Cited in over 3,000 publications, we help scientists and manufacturers gain greater insight into their studies through metabolomics. See how our approach can become a successful part of your workflow.

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