Achieve More with Precision Metabolomics™
There is a rich amount of information locked in metabolites that can lead to scientific breakthroughs and clinical insights, but sifting through massive amounts of data limits many in their quest to tap into it. Since we were founded in 2000, Metabolon has worked to develop and refine an advanced metabolomics technology for profiling the metabolites in a biological sample.
Precision Metabolomics™ is the industry-leading technology in the field of metabolomics. It combines unparalleled sensitivity and specificity with many unique, patented features to provide the broadest metabolite coverage and highest quality data possible:
- Largest reference library of authenticated, biologically relevant chemical standards
- Vast institutional knowledge & proprietary map of metabolism
- Patented bioinformatics & quality-control software
- Advanced mass spectrometry hardware
- Proprietary LIMS system
- Unparalleled quality controls throughout the process
These features collectively enable Precision Metabolomics to overcome the "signal-to-noise" challenges that plague many metabolomics platforms. Metabolon quickly and accurately identifies and quantifies metabolites and maps molecule pathways that can be used to promote wellness, identify diseases, discover biomarkers and better understand complex biological processes.
Click on the icons below to learn what makes Precision Metabolomics unique and ideal for life sciences research and product development, population health and precision medicine.
The foundation of Precision Metabolomics lies in our extensive, authenticated biochemical reference library. The biochemical library was developed by analyzing more than 3,000 pure reference standards of known metabolite structures with our LC/MS methods and cataloging all the ions that were produced.
With over 15 years of experience and more than 7,000 completed studies, Metabolon’s library now includes known and novel metabolites. We believe that this robust, ever-expanding library offers the most comprehensive metabolomics coverage available and allows for rapid metabolite identification for subsequent statistical analysis. To automatically filter extraneous, noise-producing data, our mass spectrometers are connected by proprietary software to this rigorously built chemical library.
Accurate metabolite identification is essential to delivering meaningful insights, but quite a complex task. A single sample analyzed using a mass spectrometer produces tens of thousands of data points (referred to as “ion features”), but the actual number of metabolites in the sample is typically only several hundred. This means most points are irrelevant - the result of instrument noise, process artifacts and redundant ion features. Many labs attempt to sift through those data points in a labor- and time-consuming process, which is fraught with the potential for “false positive” identification of metabolites.
To overcome this challenge, Metabolon embraces a “chemocentric approach” that rapidly and efficiently removes noise and positively identifies metabolites. Our proprietary technology automatically compares the generated data against our extensive metabolite library to isolate relevant metabolites in a matter of minutes. The result is accurate, reproducible and actionable data.
Our extensive biochemical reference library empowers patented, powerful software that makes our “chemocentric approach” to metabolomics possible. Metabolon’s innovative software efficiently identifies, quantitates and accurately quality controls the data.
Our software can identify hundreds of key metabolites within minutes of completing the analytical run by comparing the mass spectral ion features of the experimental samples to those contained in the authentic standards in our library. This elegantly designed, quality controlled software is essential to the cost-effective production of an accurate data set ready for interpretation. Chemical spectral analysts then verify the automated data analysis.
High-quality bioinformatics and data interpretation are integral to Precision Metabolomics. After all, the ultimate goal of a metabolomics study is not only to generate data, but to translate that data into biological understanding and actionable insights.
We have extensively mapped the biochemical pathways involved in metabolism, and this deep understanding has enabled us to develop powerful bioinformatics tools that allow for expert interpretation of the data we produce. Our PhD study directors leverage these tools when creating client deliverables, which may include project files, statistical data, interpreted reports, and visualization and analysis of pathways. In addition, clients can access both raw data and a variety of interpretation tools through the Client Portal, our online portal for viewing, exploring and sharing project data.
Metabolon continuously evaluates new hardware and chromatography to advance its mission to provide the highest-quality metabolomics solutions and develop innovative precision medicine products. Samples are processed using four ultra high-performance liquid chromatography/tandem accurate mass spectrometry (UHPLC/MS/MS) methods. This highly precise hardware generates the raw data that our proprietary software filters and processes to create actionable insights. All systems are operated in a rigorously controlled environment to remove as much noise from the process as possible and guided by SOPs to standardize results.
Metabolon has created a proprietary mLIMS environment custom-engineered to manage the vast number of data files generated from metabolomics studies. This secure system also allows us to track the thousands of samples we process each week and audit the data produced through our automated screenings. All data is stored safely and permanently.
mLIMS encompasses sample accessioning, sample preparation, instrumental analysis and reporting, and advanced data analysis. In fact, every step in our process is documented in mLIMS. It is customized to interface with our in-house information extraction and data visualization systems, as well as third-party instrumentation and data analysis software.
We use standardized procedures and automated liquid handler technology to ensure the highest quality sample extraction and processing. Our process is guided by rigorous SOPs, which are designed to cover a wide range of chemically diverse metabolites with very low process variability. Samples are tracked through Metabolon’s custom-built mLIMS throughout the entire process.
We employ rigorous quality control procedures across every step of our workflow. By adhering to an extensive set of SOPs, we ensure accurate metabolite identification and data quality with less than 4.5% median relative standard deviation. Our laboratories are CLIA-certified and accredited by the College of American Pathologists (CAP).
Every procedure we perform, including inter-data analysis, instrument maintenance, and reagent preparation, is governed by SOPs, so process variation is virtually non-existent. In addition, all the experimental and instrument data generated is automatically monitored by our quality-control software and then manually inspected and verified by our experienced lab personnel.