Sphingolipids Targeted Panel

Metabolon Target

Sphingolipids Targeted Panel

R Identify inflammatory markers

R Confirm proteomic changes

R Improve clinical trial planning and outcomes

About Sphingolipids

Sphingolipids are a class of lipids that have been linked to inflammatory disease states, including neurodegeneration and cardiovascular diseases. A deeper molecular understanding is needed to develop better treatments for inflammatory conditions. Sphingolipids are also involved in the maintenance of the skin barrier and therefore are routinely used in skin care products. Although sphingolipids are involved in various biological processes, they have traditionally been underrepresented in complex lipids analysis. Most sphingolipid analyses rely on genomic and proteomic readouts that provide a “first look” into upregulated or downregulated proteins but fail to confirm their activity and illuminate their final, functional role. In addition, these omics miss the impact of lipid-mediated inflammation completely and cannot give a comprehensive view of the biology of inflammation.

Other omic approaches have identified the relative presence of sphingolipids, but metabolomics​ can provide functional readouts that cannot be captured using genomic and proteomic profiling. Metabolic analysis of these analytes can confirm biological activity and identify their functional role.

The Metabolon Sphingolipid Targeted Panel is a hypothesis-generating targeted panel that measures changes in 61 lipid species across five lipid classes with strong links to inflammation. This panel extends insights from genomics and proteomics by confirming the functional impact of upregulated and downregulated genes and proteins and filling gaps in metabolic pathways and biological systems that cannot be observed with other approaches. The Sphingolipids Targeted Panel gives a final, functional readout of small molecule changes at a metabolic pathway level, including the impact of exogenous lifestyle and environmental factors. These findings can be used to study and treat inflammatory conditions by elucidating new biomarkers for drug response, disease progression, clinical outcomes, and the subtyping of disease. The Sphingolipids Targeted Panel detects known bioactive mediators of inflammation to enable you to gain insights into key disease areas such as cardiovascular, neurodegeneration, dermatitis, and more. The Sphingolipids Targeted Panel complements the Metabolon Global Discovery Panel and other inflammation-related targeted panels.  With over 20 years of experience helping commercial and academic studies gain insights from metabolomic analyses and with over 3,000 citations in scientific journals, Metabolon is leading the way in applied metabolomics solutions worldwide.

Sphingolipids Targeted Panel Details

 

Metabolites
Ceramide
C14
C16
C18
C18:1
C20
C20:1
C22
C22:1
C24
C24:1
C26
C26:1
Dihydroceramide
C14
C16
C18
C18:1
C20
C20:1
C22
C22:1
C22:2
C24
C24:1
C26
C26:1
C16
Hydroxyceramide
C14
C16
C18
C18:1
C20
C20:1
C22
C22:1
C24
C24:1
C26
C26:1
C16
Lactosylceramide
C14
C16
C18
C18:1
C20
C20:1
C22
C22:1
C24
C24:1
C26
C26:1
Sphingomyelin
C14
C16
C18
C18:1
C20
C20:1
C22
C22:1
C24
C24:1
C26
C26:1

Analysis Method and Instrumentation

LC-MS/MS (Agilent 1290 UHPLC/Sciex QTrap 5500 and 6500+)

Sample Type and Required Amounts
Sample Type Sample Requirements
Plasma 200 µL
Others on request

Disclaimer: This method is for Research Use Only and is not to be used for diagnostic purposes.

Delivering Absolute Quantification for Research and Biomarker Analysis

Our readily available or custom developed quantitative assays help you achieve your research and biomarker validation objectives with precise and fully validated methods. Our targeted assays and panels cover >1,000 metabolites and lipids across a wide range of biochemical classes, metabolic pathways, and physiological processes, and they can be customized to best fit any application.

Applications for the Sphingolipids Targeted Panel

Inflammation

The importance of inflammation in the development of multiple diseases- and health-related conditions including neurodegeneration, diabetes, cardiovascular disease, cancer, and inflammatory bowel disease is undisputed. Metabolomics can inform on inflammatory processes by providing a readout of the small molecules of an organism, tissue, biofluid, etc. This allows for direct molecular phenotyping of inflammation beyond the standard protein markers of typical assays. Metabolon offers several targeted assays, including oxysterols, fatty acids, kynurenine/tryptophan, and a range of others (central carbon, glucose tolerance, branched-chain amino acids, β-hydroxybutyrate) that can also inform on overall cardiovascular health that is often linked with inflammatory processes.

Inflammation
Cardiovascular Disease

Cardiovascular Disease

Heart failure is a leading cause of death worldwide, and there are numerous factors that lead to this and other cardiovascular diseases (CVD). Metabolomics can illuminate cardiovascular disease at multiple levels. In preclinical studies, such as with cardiomyocytes or heart tissue from model organisms, understanding mitochondrial function, energetics, and redox status can drive critical insights into disease mechanism. In human studies, metabolomics offers the opportunity to account for well-established CVD risk factors such as cholesterol and complex lipids, while simultaneously profiling thousands of other biochemicals in an unbiased fashion to enable the discovery of novel disease mechanisms and biomarkers.

Neuroscience

It is well-established that a low-carbohydrate, high-fat ketogenic diet (KD) can help treat refractory epilepsy, which affects more than a third of epileptic patients who don’t respond to existing anticonvulsive drugs. What scientists haven’t understood until recently is how this kind of diet translates to brain activity. The answer for this aspect of epilepsy lies in the gut microbiome. There are many other neurological disorders like Alzheimer’s disease, ALS, Parkinson’s disease and more. While so much remains to be understood about brain science, we do know that metabolomics is uniquely poised to understand the brain because of the ability of metabolites, small molecules, to cross the blood-brain barrier providing unique insights.

Neuroscience
Personal Care & Cosmetics

Personal Care & Cosmetics

Metabolon’s technology can provide a comprehensive survey of the skin metabolome and microbiome from a range of non-invasive skin sampling options, including our proprietary sample preparation process that allows subjects to provide samples using commercially available tape strips. In addition to our global Precision Metabolomics platform, we can quantitatively measure hundreds of lipid analytes on our pre-existing skin lipid panels. With the ability to capture biomarkers of wound repair, UV exposure, hydration, environmental exposures, and more, our proprietary platform has helped researchers understand skin conditions as diverse as dandruff, atopic dermatitis, psoriasis, acne, and aging.

Big Insights with Metabolon

Cited in over 3,000 publications, we help scientists and manufacturers gain greater insight into their studies through metabolomics. See how our approach can become a successful part of your workflow.

Contact Us

Talk with an expert

Request a quote for our services or more information on sample types and handling procedures, need a letter of support, or simply have questions about how metabolomics can advance your research.

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