C4 Single Analyte Assay
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About 7-α-Hydroxy-4-cholesten-3-one (C4)
7-α-Hydroxy-4-cholesten-3-one (C4) is an intermediate in the biosynthesis of bile acids from cholesterol. The precursor to C4 is 7α-hydroxycholesterol, which is produced from cholesterol via the hepatic enzyme, 7α-hydroxylase. 7-α-hydroxylase catalyzes the rate-limiting step in bile acid synthesis and its activity is tightly regulated via the FXR receptor. Measurement of the stable metabolite C4, a product of the next oxidative enzymatic reaction after 7-α-hydroxylase, is reflective of hepatic de novo bile acid synthesis and FXR receptor activation. Bile acid malabsorption is associated with a variety of gastrointestinal pathologies (eg, irritable bowel syndrome, ileal disease) and is characterized by elevated serum C4 levels.
Metabolomics reveals biological insights otherwise unseen. For a successful metabolomics study, both small molecule discovery and the ability to dig deeper into specific biomarkers of interest are needed to uncover actionable insights that propel new therapeutic developments. A specific combination of liquid chromatography-mass spectrometry (LC-MS) technology and biochemical expertise is required to identify this biomarker of interest and develop an assay that is sensitive enough to explore it fully.
At Metabolon, we understand the crucial role C4 plays in a number of diseases, and we’ve established best-in-class expertise for its detection. This C4 assay can be used to track biomarkers and enhance biological understanding across preclinical and clinical research.
C4 Single Analyte Assay Details
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|Sample Type and Required Amounts|
|Sample Type||Sample Requirements|
|Plasma/Serum||150 to 200 µL|
Disclaimer: This method is for Research Use Only and is not to be used for diagnostic purposes.
Delivering Absolute Quantification for Research and Biomarker Analysis
Our readily available or custom developed quantitative assays help you achieve your research and biomarker validation objectives with precise and fully validated methods. Our targeted assays and panels cover >1,000 metabolites and lipids across a wide range of biochemical classes, metabolic pathways, and physiological processes, and they can be customized to best fit any application.
C4 Single Analyte Assay Applications
Diabetes, a serious metabolic condition that results from the inability to regulate blood sugar levels, is a worldwide epidemic. According to the Diabetes Research Institute Foundation, more than 30 million Americans and 422 million people worldwide have been diagnosed with diabetes. Yet much remains unknown about how diabetes develops, why certain individuals are at increased risk, and whether the metabolic dysregulation can be corrected rather than merely managed.
Metabolomics enables simultaneous assessment of hundreds of compounds present in living systems, making it a critical tool that advances our understanding of disease mechanisms and opens doors to identify novel targets and treatments for conditions like diabetes.
Big Insights with Metabolon
Cited in over 3,000 publications, we help scientists and manufacturers gain greater insight into their studies through metabolomics. See how our approach can become a successful part of your workflow.
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