Shorten Development Cycles and Improve Efficiency

Metabolomics is a direct measure of a system’s active biology, assessing all complex factors that can influence the production process, including genetic, environmental, and nutritional influences. Moreover, metabolomic profiling can be performed to evaluate both cells and media. This increases the assessment of active biology by providing a view of what is happening within the cell as well as what is being released to the media.

Metabolon’s proprietary metabolomics technology provides the broadest coverage of metabolites in the industry, so you can have confidence in your bioprocess monitoring. While traditional technologies focus on a conventional set of 10 to 20 biomarkers, Metabolon can routinely detect 500 to 1500 metabolites in cell culture systems, including amino acids, carbohydrates, cofactors, vitamins, nucleotides, and lipids. Therefore, Metabolon can expand the assessment of biochemical space and provide you with greater understanding and more opportunities for optimization. Our metabolomic approach provides comprehensive coverage of the metabolome and accurate identification of metabolites with high precision. Another challenge of metabolomics profiling is being able to derive actionable knowledge from the data. Our analytical capabilities are supported by informatics research and an extensive knowledge base of metabolism, which enables interpretation of the data and generation of knowledge.

β-Hydroxybutyrate (BHB, 3-hydroxybutyrate) is an endogenous ketone body that accumulates during periods of fasting, calorie restriction and prolonged exercise. It is created via a multi-step process involving the break-down of fatty acids into acetyl CoA, conversion to acetoacetate and reduction to β-hydroxybutyrate in the liver. BHB is the primary ketone found in the blood and is necessary for brain function especially when glucose is unavailable. It also provides neuroprotective benefits, such as relieving oxidative stress and inhibition of apoptotic pathway in cells.

Impaired Glucose Tolerance is a prediabetic state of hyperglycemia that is associated with insulin resistance and an increased risk of cardiovascular pathology (Barr, 2007). The condition occurs when blood glucose levels remain high for an extended period after oral ingestion of glucose but not high enough to be diagnosed as type 2 diabetes.

Impaired Glucose Tolerance can be assessed with a single fasted blood draw by measuring a panel of selected metabolites comprised of two small organic acids (α-hydroxybutyric acid (AHB) and 4-methyl-2-oxopentanoic acid (4MOP)), 2 lipids (oleic acid and linoleoyl glycerophosphocholine (LGPC)), a ketone body (β-hydroxybutyric acid (BHBA)), an amino acid (serine), a vitamin (pantothenic acid (vitamin B5)), and glucose.