| Plate format | 96-well plate |
| Sample wells per plate | 84 wells |
| QC wells per plate | 12 (8 Kit QC + 4 blanks) |
| Minimum project size | 15 samples |
| Maximum project size | 1,512 samples / 18 plates |
| Multi-project per plate | Supported |
| Supported sample types | Plasma (EDTA), Serum, Urine More to follow |
| Extraction method | All extraction protocols for each matrix provided (includes recovery standards) |
| Reconstitution solution | Contains 11 standards for each mode |
| High-molecular weight internal standard | Included |
| Shipping | Room temperature |
| Storage | 4° C |
| Automation compatible | Yes |
| LC platform | Waters Acquity I-Class UPLC Thermo Vanquish Flex or Horizon |
| Mass spec platform | Thermo Scientific Orbitraps
More instruments to follow |
| Software required | Xcalibur 4.2 or higher Freestyle 1.8 or higher Tune Software 2.11 or higher |
| Analysis platform | Integrated Bioinformatics Platform (web-based) |
| Column (not included) | Waters BEH C18 (2.1 × 100 mm, 1.7 µm) column is required for this kit (not included) |
| Parameter | Specification | Status |
|---|---|---|
| Metabolite search list | ~1,500 metabolites | Validated * |
| Plasma readout | > 700 metabolites | Validated * |
| Urine readout | > 700 metabolites | Validated * |
| Biological sub-pathways covered | 100+ pathways searched 80+ pathways detected | Validated * |
| Identification level | 80% Level 1, 20% Level 2 | Library-confirmed |
| Confidence Score Lenses | Tailor coverage and accuracy | Unique to Metabolon |
* Tested across six independent laboratories
The QA/QC framework begins with a System Suitability Test (SST), which confirms instrument readiness prior to sample processing. This encompasses verification of extraction solvent composition, column configuration, instrument calibration, and correct method file setup. Following plate acquisition, a Plate QC report is generated that evaluates run quality across key parameters including retention time deviation, instrument sensitivity, and mass accuracy.
| QC Measure | Specification | Metric |
|---|---|---|
| System Suitability Test (SST) | Pre-extracted pooled human plasma | Results in ~30 min |
| Plate QC (PQC) | Lyophilized plasma co-extracted with samples | Results in ~2 hours |
| Internal standards (positive and negative mode) | 11 per mode; retention, peak shape, ionization monitoring | < 10% RSD |
| Workflow | Specification | Time |
| Hands-on time | Include instrument setup, samples extraction and SST/PQC review | 10 hours |
| Instrument time | Positive ionization 24hrs Negative ionization 24hrs | 48 hours |
| Full workflow | From sample extraction to data insight | 5 days |
* Tested across six independent laboratories
| LC platform | Waters Acquity I-Class UPLC Thermo Vanquish Flex or Horizon |
| Mass spec platform | Thermo Scientific Orbitraps
|
| Software required | Xcalibur 4.2 or higher Freestyle 1.8 or higher Tune Software 2.11 or higher |
| Column (not included) | Waters BEH C18 (2.1 × 100 mm, 1.7 µm) column is required for this kit (not included) |
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