Fatty Acid Metabolism Targeted Panel

Metabolon Target

Fatty Acids Metabolism Targeted Panel

R 28 Metabolites

R Absolute Quantitation

R Rigorous Quality Control

R End-to-end Service

Fatty Acids Metabolism

Fatty acids play many physiologically important roles in an organism. They are not only key metabolites of energy storage and production but also the basic building blocks of complex lipids that form cellular membranes. A variety of bioactive forms of fatty acid metabolites, known as lipid mediators, act as local hormones and are involved in many physiological systems and pathological processes (e.g., eicosanoids, lysophospholipids, resolvines, protectins, maresines). Dysregulation of fatty acid metabolism has been associated with many diseases.

Metabolomics reveals biological insights otherwise unseen. In a successful metabolomics study, both small molecule discovery and the ability to dig deeper into specific biomarkers of interest can uncover actionable insights that propel new therapeutic developments. Liquid chromatography-mass spectrometry (LC-MS) technology and expertise are required to identify these biomarkers of interest and develop assays that are sensitive enough to explore them fully.

At Metabolon, we understand the crucial role that fatty acids play in disease, and we’ve established best-in-class expertise. This panel focuses on specific fatty acids and their metabolic pathways and can be used to track biomarkers and enhance biological understanding across preclinical and clinical research.

Fatty Acids Metabolism Targeted Panel Details

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LLOQ*
Metabolite Plasma/Serum
Myristic Acid (14:0) 1.00 µg/mL
Pentadecanoic Acid (15:0) 0.600 µg/mL
Palmitic Acid (16:0) 8.00 µg/mL
Stearic Acid (18:0) 4.0 µg/mL
Arachidic Acid (20:0) 1.00 µg/mL
Behenic Acid (22:0) 1.00 µg/mL
Lignoceric Acid (24:0) 0.600 µg/mL
Myristoleic Acid (14:1n5) 0.600 µg/mL
Palmitoleic Acid (16:1n7) 1.00 µg/mL
Vaccenic Acid (18:1n7) 1.00 µg/mL
Oleic Acid (18:1n9) 8.00 µg/mL
Cis-11-Eicosaenoic Acid (20:1n9) 0.600 µg/mL
Erucic Acid (22:1n9) 0.600 µg/mL
Nervonic Acid (24:1n9) 1.00 µg/mL
Mead Acid (20:3n9) 1.00 µg/mL
Linoleic Acid (18:2n6) 8.00 µg/mL
Gamma-Linolenic Acid (18:3n6) 0.600 µg/mL
Dihomo-Gamma-Linolenic Acid (20:3n6) 1.00 µg/mL
Arachidonic Acid (20:4n6) 6.00 µg/mL
Adrenic Acid (22:4n6) 0.600 µg/mL
Osbond Acid (22:5n6) 0.600 µg/mL
Cis-11,14-Eicosadienoic Acid (20:2n6) 1.00 µg/mL
Alpha-Linolenic Acid (18:3n3) 0.600 µg/mL
Stearidonic Acid (18:4n3) 0.600 µg/mL
Eicosatetraenoic Acid (ETA) (20:4n3) 0.600 µg/mL
Eicosapentaenoic Acid (EPA) (20:5n3) 1.00 µg/mL
Docosapentaenoic Acid (22:5n3) 1.00 µg/mL
Docosahexaenoic Acid (DHA) (22:6n3) 2.00 µg/mL
*Lower Limit of Quantitation (LLOQ) varies for each sample type.

Analysis Method and Instrumentation:
Fatty acids are determined by GC-MS (Agilent 7890A/5975C) as their respective methyl esters after conversion of all free and conjugated fatty acids into methyl esters (FAME Analysis). The Fatty Acids Metabolism Targeted Panel measures the total fatty acid content of 28 fatty acids in a variety of matrices.

Sample Type and Required Amounts
Sample Type Sample Requirements
Plasma/Serum 150 to 250 µL
Others on request

Disclaimer: This method is for Research Use Only and is not to be used for diagnostic purposes.

Delivering Absolute Quantification for Research and Biomarker Analysis

Our readily available or custom developed quantitative assays help you achieve your research and biomarker validation objectives with precise and fully validated methods. Our targeted assays and panels cover >1,000 metabolites and lipids across a wide range of biochemical classes, metabolic pathways, and physiological processes, and they can be customized to best fit any application.

Fatty Acids Metabolism Targeted Panel Applications

Nutrition

Metabolomics can be essential for investigating the association between nutrition and health status, as metabolites represent a functional readout at the interface between diet and the complex metabolic systems that influence both health and disease. By illuminating the interactions of metabolites throughout the body within defined pathways, targeted metabolomics can help investigators gain new insights into the absorption and digestion of diet-derived macronutrients, monitor metabolism by various organ systems, and subsequently assess the underlying biology of various health and disease states.
Nutrition
Cardiovascular Disease

Cardiovascular Diseases

Heart failure is a leading cause of death worldwide, and there are numerous factors that lead to this and other cardiovascular diseases (CVD). Metabolomics can illuminate cardiovascular disease at multiple levels. In preclinical studies, such as with cardiomyocytes or heart tissue from model organisms, understanding mitochondrial function, energetics, and redox status can drive critical insights into disease mechanism. In human studies, metabolomics offers the opportunity to account for well-established CVD risk factors such as cholesterol and complex lipids, while simultaneously profiling thousands of other biochemicals in an unbiased fashion to enable the discovery of novel disease mechanisms and biomarkers.

Diabetes

Diabetes is a serious metabolic condition affecting more than 37 million Americans and 460 million people worldwide according to the most recent report from the Centers for Disease Control. Despite being a worldwide epidemic, much remains unknown about individual risk factors for diabetes development, and research is currently being done to identify new and effective treatment for diabetes at all stages. By facilitating assessment of specific metabolic pathways impacted by diabetes, targeted metabolomics can be a critical tool used to identify biomarkers of disease development for early intervention and novel targets to control disease progression, as well for the development of new pharmaceuticals with specific mechanisms of action.
Diabetes
Cancer

Cancer

Dysregulated metabolism, especially glucose and biosynthetic pathways, is a hallmark change and is essential for the growth and proliferation of individual cancer cells; indeed, the physiological health of the patient is as much a part of the equation as the tumor. Metabolomics can identify cancer-specific drug targets and broadly assess the patient’s overall metabolic phenotype, addressing key questions regarding who might respond to the therapy and who may be adversely affected by a given treatment. This can give insight into determinants of responders/non-responders and potential factors that can possibly cause adverse events, thereby streamlining the screening process, and aiding in better patient care. Thus, Metabolon’s targeted metabolite assays for amino acids, central carbon, glucose tolerance, fatty acid, and tryptophan/kynurenine ratio can inform on treatment development and position programs for success by providing a functional readout of the patients’ and the tumors’ molecular phenotype in multiple biochemical pathways.

Neuroscience

Metabolomics provides valuable insight into the realm of brain science. Through dynamic bidirectional communication along the “gut-brain axis,” gut microbes collaborate with their hosts to regulate the development and function of the nervous system. For instance, in epilepsy, it is well-established that a low-carbohydrate, high-fat ketogenic diet (KD) can help treat refractory epilepsy, which affects more than a third of epileptic patients who don’t respond to existing anticonvulsive drugs. What scientists haven’t understood until recently is how this kind of diet translates to brain activity. The answer for this aspect of epilepsy lies in the gut microbiome. There are many other neurological disorders like Alzheimer’s disease, ALS, Parkinson’s disease and more. While so much remains to be understood about brain science, we do know that metabolomics is uniquely poised to understand the brain because of the ability of metabolites, small molecules, to cross the blood-brain barrier providing unique insights.
Neuroscience

Big Insights with Metabolon

Cited in over 3,000 publications, we help scientists and manufacturers gain greater insight into their studies through metabolomics. See how our approach can become a successful part of your workflow.

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