Nieman, D C, et al. Metabolomics Approach to Assessing Plasma 13- and 9-Hydroxy-Octadecadienoic Acid and Linoleic Acid Metabolite Responses to 75-Km Cycling. Am J Physiol Regul Integr Comp Physiol, 2014. 307 (1):R68-74.


Biomarkers to monitor stress, workout recovery and efficacy in the field of exercise science are a valuable tool for the development of precision training. Although there are established markers such as F2-isoprostanes, some regard them as time consuming and expensive because of lack of stability and trace abundance. Thus investigators screened cyclists before and after a bout of intense cycling with metabolomics to reveal a profound increase in 3-HODE + 9-HODE. Significantly, these markers associated strongly with the well established F2-isoprostanes but are more stable and 20 times more abundant. Further, the HODEs also translate to different forms of intense exercise as shown in previous work with runners. This is yet another example of how many established biomarkers are under the classic “lamp post” and that metabolomics offers ample opportunity to find new biomarkers that can foster research advancement.

Metabolon results led to:

  • Biomarkers that improve the gold standard markers for oxidative stress in intense exercise


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